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Objective@# To explore the application of digital technology in the restoration of partial edentulous patients with microstomia. @*Methods@# A patient with microstomia was presented and seeking for the restoration of her full edentulous in the upper jaw and partial edentulous in the lower jaw (Kennedy Ⅲ). A digital intraoral scanning was used to obtain digital impressions of soft and hard tissues in the oral cavity. Computer aided design and 3D printing technology were used to design and fabricate the metal framework. @* Results @#The patient had no difficulty to wear or take off the dentures. The maxillary and mandibular dentures showed good retention, stability, mastication function and articulation. There was no tenderness in the one week and one month′s follow-up. And the chewing efficiency was satisfactory@*Conclusion@#This case report successfully designed and fabricated mandibular removable partial dentures for patients with microstomia through intraoral scanning and 3D printing technology. Thus, this work provides a new method and idea for treating partial edentulous dentition with microstomia
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OBJECTIVES: To explore the effects of serum from patients with ankylosing spondylitis on the canonical Wnt/β-catenin pathway and to assess whether the serum has an osteogenic effect in MG63 cells. METHODS: MG63 cells were cultured with serum from 45 ankylosing spondylitis patients, 30 healthy controls, or 45 rheumatoid arthritis patients. The relative PPARD, fra-1, MMP7, OPG and RANKL mRNA levels were measured using quantitative real-time polymerase chain reaction. Associations between gene expression and patient demographics and clinical assessments were then analyzed. RESULTS: MG63 cells treated with serum from ankylosing spondylitis patients had higher PPARD, fra-1, MMP7 and OPG gene expression than did cells treated with serum from controls or rheumatoid arthritis patients (all p<0.05). RANKL expression was higher in MG63 cells treated with serum from patients with ankylosing spondylitis or rheumatoid arthritis than in those treated with serum from controls (both p<0.05). The OPG/RANKL ratio was also higher in MG63 cells treated with serum from ankylosing spondylitis patients than in those treated with serum from controls (p<0.05). No associations were found between the expression of the five genes and the patient demographics and clinical assessments (all p>0.05). CONCLUSIONS : Serum from ankylosing spondylitis patients increases PPARD, fra-1, MMP7, OPG and RANKL expression and the OPG/RANKL ratio in MG63 cells; these effects may be due to the stimulatory effect of the serum on the Wnt pathway.